Coherent Raman Scattering Microscopy in Biology and Medicine

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Coherent Raman Scattering Microscopy in Biology and Medicine.

Advancements in coherent Raman scattering (CRS) microscopy have enabled label-free visualization and analysis of functional, endogenous biomolecules in living systems. When compared with spontaneous Raman microscopy, a key advantage of CRS microscopy is the dramatic improvement in imaging speed, which gives rise to real-time vibrational imaging of live biological samples. Using molecular vibrat...

متن کامل

Coherent anti-stokes Raman scattering microscopy: chemical imaging for biology and medicine.

Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free imaging technique that is capable of real-time, nonperturbative examination of living cells and organisms based on molecular vibrational spectroscopy. Recent advances in detection schemes, understanding of contrast mechanisms, and developments of laser sources have enabled superb sensitivity and high time resolution. Emergi...

متن کامل

Polarization coherent anti-Stokes Raman scattering microscopy.

We report polarization coherent anti-Stokes Raman scattering (P-CARS) microscopy that allows vibrational imaging with high sensitivity and spectral selectivity. The nonresonant background signals from both Raman scatterers and the solvent are efficiently suppressed in P-CARS microscopy. We demonstrate P-CARS imaging of unstained cells based on the contrast of the protein amide I band.

متن کامل

Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology.

Laser-scanning coherent anti-Stokes Raman scattering (CARS) microscopy with fast data acquisition and high sensitivity has been developed for vibrational imaging of live cells. High three-dimensional (3D) resolution is achieved with two collinearly overlapped near infrared picosecond beams and a water objective with a high numerical aperture. Forward-detected CARS (F-CARS) and epi-detected CARS...

متن کامل

Refractive effects in Coherent Anti-Stokes Raman Scattering (CARS) Microscopy

Coherent anti-Stokes Raman scattering (CARS) microscopy with high sensitivity, and high three dimensional resolution, has been developed for vibrational imaging of chemical species [1]. Due to the coherent nature of the CARS emission, it has been reported that the Epi-CARS (E-CARS) and Forward-CARS (F-CARS) signals detection depends on the size and shape of the sample [2,3]. In this paper, we i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Annual Review of Biomedical Engineering

سال: 2015

ISSN: 1523-9829,1545-4274

DOI: 10.1146/annurev-bioeng-071114-040554